SIEN-NET™: Custom capture technology

 

SIEN-NET is an advanced capture platform that enables rapid capture and detection of multiple targets of interest in remarkably small sample volumes. As seen in the micro-scale image on the left, SIEN-NET utilizes a unique 3-D scaffold to bind targets of interest with exceptional yield and specificity, while avoiding unwanted contamination. SIEN-NET is adaptable for single or multi-target evaluation of virtually any molecule or even cell type of interest, often surpassing sensitivities of pre-existing assays to the femto-molar range. The technology is compatible with a wide variety of downstream applications, and raw sample compatibility allows for sample prep times of less than 30 minutes.

The SIEN-NET™ advantage

Efficiency


SIEN-NET technology benefits from unparalleled capture and detection sensitivities, combined with minimized non-specific binding. Sample prep times are typically 30-45 minutes from raw sample to answer.

Conventional Capture Methods

  • Capture 1 target of interest
  • Low capture yield per bead
  • High non-specific binding
  • Extensive sample prep time

SIEN-NET™

  • Simultaneous capture of multiple targets of interest
  • Maximum binding capacity per bead
  • 30-45 minute sample-to-result
  • Point-of-Care enabling

Customisability


SIEN-NET is highly customisable to bind and extract a wide variety of cellular and molecular targets from almost any sample type.

Compatible Samples

Clinical Targets

  • Whole Blood
  • Plasma
  • Serum
  • Saliva
  • Nasal Swabs
  • Throat Swabs
  • Skin Swabs
  • Faeces
  • Cells
  • Protein
  • Nucleic Acid
  • Lipids
  • Whole Virus
  • Carbohydrates
  • Vesicles
  • Small Molecules

Flexibility


Once target(s) are captured, SIEN-NET remains compatible with a variety of downstream clinical, R&D and industrial applications.

Clinical Test Applications

  • FACS
  • FISH
  • Next Gen Sequencing
  • Cellular Activity Assays
  • ELISA
  • PCR (q & qRT)
  • Mass Spectrometry
  • Luminex
  • Fluorimetry
  • Colorimetric Assays
  • Fluorescent Microscopy
  • Plasmon Resonance

Innovative Science


Dr Emily Stein

Research & Development Manager

Emily is the inventor of SIEN-NET technology and is based in Sienna’s USA headquarters in Minneapolis. Emily brings a unique scientific background to Sienna that spans molecular biology, microbiology and molecular & cellular immunology. She completed her Post-Doctoral Fellowship in Immunology & Rheumatology at Stanford University; she holds a PhD in Microbiology from the University of California at Berkeley, and a Bachelor of Science in Microbiology and Immunology from the University of Iowa.

Emily is leading the development of the SIEN-NET technology and working with her colleagues to complete the development and design a manufacturing process for the product.

Compatible targets include many that are key biomarkers in cutting-edge diagnostic tests such as exosomes, cells, proteins, nucleic acids and lipids. - Dr Emily Stein

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